Seminar : Estimation of Gene Expression Using Multiple Laser Scans of Microarrays
April 9, 2007 – 2:01am
Full Title:
Statistical Estimation of Gene Expression Using Multiple Laser Scans of Microarrays
Speaker:
Mizanur R Khondoker, PhD
Departement of Statistics, University of Dhaka, Bangladesh
Date/Time:
Monday, April 9, 2007, 1200
Venue:
ISRT Seminar Room
ABSTRACT
We propose a statistical model for estimating gene expression using data from multiple laser scans at different settings of hybridized microarrays. A functional regression model is used, based on a non-linear relationship with both additive and multiplicative error terms. The function is derived as the expected value of a pixel, given that values are censored at 65535, the maximum detectable intensity for double precision scanning software. Maximum likelihood estimation based on a Cauchy distribution is used to fit the model, which is able to estimate gene expressions taking account of outliers and the systematic bias caused by signal censoring of highly expressed genes. We have applied the method to experimental data. Simulation studies suggest that the model can estimate the true gene expression with negligible bias.
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Mailing Address
Director,
Institute of Statistical Research and Training (ISRT)
University of Dhaka
Dhaka 1000, Bangladesh